Banca de QUALIFICAÇÃO: REILA FERREIRA DOS SANTOS

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : REILA FERREIRA DOS SANTOS
DATE: 19/04/2024
TIME: 14:00
LOCAL: https://meet.google.com/uto-idco-tra
TITLE:

ESTUDO FENOLÓGICO E CULTIVO IN VITRO DE EMBRIÕES DE 73 CAJARANEIRA-ANÃ (Spondias dulcis Parkinson)

KEY WORDS:

Reproductive biology. Embryo rescue. Micropropagation. Biotechnology.

PAGES: 35
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUBÁREA: Fitotecnia
SPECIALTY: Produção de Mudas
SUMMARY:

This thesis aimed to characterize the reproductive phenology and study the in vitro performance  of cajaraneira (Spondias dulcis Parkinson) embryos cultivated in the technological showcase  of Embrapa Roraima in the municipality of Boa Vista-RR. This work arose from the need to  contribute to the propagation process of the species, since in the natural environment there is a  slow and uneven germination, which is an obstacle to making cultivation viable. To this end, it  was necessary to previously carry out a study on the phenological behavior of the species,  focusing on the moment in which the turgidity of vegetative buds began after pruning until the  emergence of reproductive buds and the moment of harvest. This research makes up the first  article of the Thesis and provides essential information for carrying out the following  experiment, which concerns the age at which the fruits are collected with the possibility of  finding viable embryos for in vitro cultivation. In the second article, cajaraneira embryo  cultivation tests were carried out in-vitro, under different culture media (WPM and MS) and  phenological age. It was observed that cultivated cajaraneira plants presented seven of the 10  stages described on the BBCH scale, with stage 9 (senescence) when it was not observed due  to irrigation management. Plants require 3840 degree days to complete all stages of growth.  The understanding of the phenological stages presented will provide a standardization of the  phenological stages for S. dulcis in addition to providing the important stages for planning the  cultural practices necessary during the cultivation of the species. Together with the description  of the BBCH phenological scale, it was possible to describe the unit of accumulated heat in  degree days, which will be a parameter for evaluating climate change and testing the adaptability of genotypes to different environmental conditions. Regarding in vitro cultivation,  it is concluded that although no statistical differences were observed for germination, visually,  embryos 120 days had the best percentage of germination (55%). Therefore, due to the gain in  collection time; greater ease of excising embryos; positive correlation with germination due to  their smaller size and satisfactory in vitro development (aerial and root parts), this age (120 167 days) presented potential for embryo propagation. Both culture media provided embryo  germination. However, adjustments to in vitro establishment protocols are necessary in order  to minimize contamination and loss of embryos. Furthermore, further studies must consider the  genetic differences between plants, since they originate from propagation via seminifera and  have a direct influence on the estimation of the number of fruits needed to obtain 20 germinated 172 embryos.

COMMITTEE MEMBERS:
Externo à Instituição - AURELIO RUBIO NETO - UFG
Interno - ***.965.779-** - DANIEL AUGUSTO SCHURT - EMBRAPA
Presidente - ***.784.132-** - EDVAN ALVES CHAGAS - EMBRAPA
Externo ao Programa - 3327379 - RICARDO MANUEL BARDALES LOZANO - nullInterno - 1030577 - WELLINGTON FARIAS ARAUJO